CD28-independent, TRAF2-dependent costimulation of resting T cells by 4-1BB ligand

by Catherine Saoulli

Publisher: National Library of Canada in Ottawa

Written in English
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Thesis (M.Sc.) -- University of Toronto, 1998.

CDindependent, TRAF2-dependent costimulation of resting T cells by 4- 1BB ligand. J. Exp. Med. ; View in Article. Triggering of BB on CD8 + and CD4 + T cells fostered IL-2 production in a CDindependent manner []. Engagement of BB paralleled by blocking of programmed cell death protein 1 (PD T cell survival in a CDindependent manner TRAF2-dependent costimulation of resting T cells. human CD T cells by BB ligand. Eur J Immunol. Summary The tumour necrosis factor (TNF) ligands CD, CD70 and TNF receptors CD and CD are all involved in allograft rejection. Because these molecules are not present on resting T cells, w.

CDindependent, TRAF2-dependent costimulation of resting T cells by 4–1BB ligand. J Exp Med. ; – Crossref Medline Google Scholar; Ria M, Eriksson P, Boquist S, Ericsson CG, Hamsten A, Lagercrantz J. Human genetic evidence that OX40 is implicated in myocardial infarction. Biochem Biophys Res Commun. ;

CD28-independent, TRAF2-dependent costimulation of resting T cells by 4-1BB ligand by Catherine Saoulli Download PDF EPUB FB2

CDindependent, TRAF2-dependent costimulation of resting T cells by BB ligand. Saoulli K(1), Lee SY, Cannons JL, Yeh WC, Santana A, Goldstein MD, Bangia N, DeBenedette MA, Mak TW, Choi Y, Watts TH. Author information: (1)Department of Immunology, and Amgen Institute, University of Toronto, Toronto, Ontario M5S 1A8, by: A number of studies have shown a role for BB in T cell activation using either transfected ligand (), antibodies against the BB molecule (6, 12–14), or blocking studies with a soluble form of the BB receptor (10, 11, 15, 16).Anti–BB antibodies are effective in the activation of T cells that have been preactivated via their TCR to induce high levels of BB receptor expression Cited by: Comparison of immobilized sBBL with immobilized anti-CD28 for costimulation of IL-2 production by CD28 and CD28 T cells.

Resting T cells isolated from CD28 or CD28 T cells were stimulated by. BibTeX @MISC{Ligand_cdindependent,traf2-dependent, author = {T Cells -bb Ligand and Katina Saoulli and Soo Young Lee and Jennifer L. Cannons and Wen Chen Yeh and Angela Santana and Marni D. Goldstein and Naveen Bangia and Mark A.

Debenedette and Tak W. Mak and Yongwon Choi and Tania H. Watts}, title = {CDindependent, TRAF2-dependent Costimulation of Resting}, year = {}}. However, when BB ligand is provided together with strong TCR signals, then BBL and anti-CD28 are equally potent in stimulation of IL-2 production by resting T cells.

We find that TNF receptor-associated factor (TRAF)1 or TRAF2 associate with a glutathione S-transferaseBB cytoplasmic domain TRAF2-dependent costimulation of resting T cells by 4-1BB ligand book protein in vitro.

CDindependent, TRAF2-dependent Costimulation of Resting T Cells by BB Ligand By Katina Saoulli, Soo Young Lee, Jennifer L. Cannons, Wen Chen Yeh, Angela Santana, Marni D.

Goldstein, Naveen Bangia, Mark A. DeBenedette, Tak W. Mak, Yongwon Choi and Tania H. Watts. However, when BB ligand is provided together with strong TCR signals, then BBL and anti-CD28 are equally potent in stimulation of IL-2 production by resting T cells.

We find that TNF receptor–associated factor (TRAF)1 or TRAF2 associate with a glutathione S-transferase–BB cytoplasmic domain fusion protein in vitro. In addition to B7-CD28 and related ligand-receptor interactions, the TNF family-TNF receptor (TNFR) family interactions are a rich source of costimulation for T-cell activation.

9 BB (CD) is a type I membrane protein of the TNFR family and functions as a costimulatory molecule in T cells (reviewed in Kwon et al 10). BB is expressed. BB (CD) is a costimulatory member of the TNFR family expressed on activated T cells.

Its ligand, BBL, is expressed on activated APC. In the mouse, CD8 T cells are preferentially activated by agonistic anti-murine BB Abs. However, murine BBL can stimulate both CD4 and CD8 T cells. To date, there are only limited data on the effects of BBL on human T cell responses.

CDindependent, TRAF2-dependent costimulation of resting T cells by BB ligand. Saoulli K, Lee SY, Cannons JL, Yeh WC, Santana A, Goldstein MD, Bangia N, DeBenedette MA, Mak TW, Choi Y, Watts TH. J Exp Med, (11), 01 Jun CDindependent, TRAF2-dependent costimulation of resting T cells by BB ligand.

J Exp Med. ; View in Article. BB Ligand. BB (CD/ILA: receptor induced by lymphocyte activation), another member of the TNF family, is a co-stimulatory molecule expressed on activated T cells (Kwon and Weissman, ). BB ligand (BBL) is an inducible molecule present on several APC types, including B cells, macrophages and DCs (Pollock et al., Arch, R.H., and Thompson, C.B.,BB and Ox40 are members of a tumor necrosis factor (TNF)-nerve growth factor receptor subfamily that bind TNF receptor-associated factors and activate nuclear factor kappaB, Mol Cell Biol 18(1)– PubMed Google Scholar.

CD is expressed by activated T cells of both the CD4+ and CD8+ lineages. Although it is thought to function mainly in co-stimulating those cell types to support their activation by antigen presenting cells expressing its ligand (CDL), CD is also expressed on dendritic cells, B cells, NK cells, neutrophils and macrophages.

Costimulation induces T cell proliferation and inhibition of AICD. (a) Measurement of CD80 and 4–1BBL expression on stably transfected TE cells, i.e. 80 or TE.4–1BB. TE cells were stained with a PE‐labeled anti‐CD80 antibody or the PE‐labeled anti‐4–1BBL antibody (solid lines). CD (4‐1BB, tnfsfr9) was originally reported by the group of B.

Kwon in as a cDNA clone whose sequence showed homology to TNF receptors and as being selectively expressed in activated versus resting T cells 1, 2. BB ligand receptor.

CDw T-cell antigen BB homolog. T-cell antigen ILA. CD_antigen: CD Gene names i: Name:TNFRSF9. Synonyms: CD, ILA. Organism i: Homo sapiens (Human) Taxonomic identifier i: Taxonomic lineage i › Eukaryota › › Metazoa › › › › Chordata › Craniata › Vertebrata › › › Euteleostomi. T cell proliferation and suppression assays.

The effect of soluble SABBL protein on of CD8 + T cell proliferation was determined as previously described ().For suppression assay, CD4 + CD25 + Treg and CD4 + CD25 − Teff cells were sorted from WT and BB KO C57BL/6 mice by flow cytometry.

Treg cells were cocultured at various ratios with a fixed number of Teff cells (×10 4 cells. Saoulli K, Lee SY, Cannons JL, Yeh WC, Santana A, Goldstein MD, Bangia N, DeBenedette MA, Mak TW, Choi Y, Watts TH () CDindependent, TRAF2-dependent costimulation of resting T cells by BB ligand.

J Exp Med – CrossRef PubMed Google Scholar. On T cells, BB is transiently expressed after T-cell receptor engagement and, when BB is engaged by the natural or artificial ligand, provides CDindependent costimulation resulting in enhanced proliferation and Th1 cytokine production.

11,12 The major biological ligand, BBL, is expressed on activated professional antigen presenting. K Saoulli, SY Lee, JL Cannons, WC Yeh, A Santana, MD Goldstein, et pendent, TRAF2-dependent costimulation of resting T cells by BB ligand J Exp Med, (), pp.

View Record in Scopus Google Scholar. M.A. DeBenedette, T. Wen, M.F. Bachmann, P.S. Ohashi, B.H. Barber, K.L. Stocking, J.J. Peschon, T.H. WattsAnalysis of BB ligand-deficient mice and of mice lacking both BB ligand and CD28 reveals a role for BB ligand in skin allograft rejection and in the cytotoxic T cell response to influenza virus.

CDindependent, TRAF2-dependent costimulation of resting T cells by BB ligand. Exp. Med. ; View in Article. DeBenedette MA, Shahinian A, Mak TW, Watts TH.

Costimulation of CD T lymphocytes by BB ligand. J Immunol. ; (2) (Biology) Hurtado JC, Kim SH, Pollok KE, Lee ZH, Kwon BS. Potential role of BB in T cell activation. Comparison with the costimulatory molecule CD J Immunol.

; (7) (Biology) Rev. Saoulli K, Lee SY, Cannons JL, et al. CDindependent, TRAF2-dependent costimulation of resting T cells by BB ligand. J Exp Med.

; [ PMC free article ] [ PubMed ]. CD (BB), is an inducible T-cell costimulatory receptor and a member of the tumor necrosis factor receptor (TNFR) superfamily. It is expressed on activated T cells and activated natural killer (NK) cells, but is constitutively expressed on a population of splenic dendritic cells, (DCs).

The natural counter receptor for CD is BB ligand, a member of the TNf superfamily that is weakly. CDindependent, TRAF2-dependent costimulation of resting T cells by 4–1BB ligand. Costimulation through the CD/BB pathway protects human melanoma tumor-infiltrating lymphocytes from activation-induced cell death and enhances antitumor effector function.

Therefore, the abundance of diBB on the T cell surface may be correlated with the reactivity of T cells and even with the treatment efficacy of BB-specific agonist mAbs in tumor immunotherapy.

The binding of utomilumab can subsequently block the interaction between BB and BBL, preventing ligand-induced co-stimulatory signaling. A model for BB-induced effector responses regulating autoimmunity.

At the top, a DC (yellow) presents antigen to a CD8 T cell (blue). Input of agonist antiBB mAb enforces BB co-stimulation and the CD8 T cells become conditioned to express effector function (left-hand side) and regulate autoimmunity (right-hand side).

Saoulli K, Lee SY, Cannons JL, et al. CDindependent, TRAF2-dependent costimulation of resting T cells by BB ligand. J Exp Med ; – OpenUrl Abstract / FREE Full Text. In vitro expansion and CD expression kinetics on blood‐derived T cells upon bead‐induced activation. Peripheral blood mononuclear cells (PBMCs) of a single healthy donor were divided into three identical aliquots.

In vitro stimulations were then performed with CD3/CD28 or CD3/CD28/CD beads at a bead/cell ratio of with IU/ml IL‐2 added and were compared. The 4‐1BB/4‐1BBL pathway has been well defined as a costimulatory pathway for different T‐cell subsets, leading to a variety of immune responses [6, 19].

Studies with 4‐1BBL+ APC have shown that interaction of 4‐1BB with its ligand stimulates cell proliferation and production of IL‐2 and IL‐4 by CD4 T cells.Saoulli K, Lee SY, Cannons JL, et al: CDindependent, TRAF2-dependent costimulation of resting T cells by BB ligand.

J Exp Med ;– PubMed.